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Information
| Code | BB625 |
| Name | Ultra-Cold Conditions (Cryopreservation) Preservation Methods and Applications |
| Term | 2022-2023 Academic Year |
| Term | Spring |
| Duration (T+A) | 2-2 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. YILDIZ KAÇAR |
| Course Instructor |
1 |
Course Goal / Objective
This course aims to teach the basic concepts and subjects of cryopreservation.
Course Content
The contents of this course include introduction to the historical development of cryopreservation techniques; slow cooling techniques; rapid cooling techniques; cold hardening, cryoprotectants (DMSO, ethylene glycol, sucrose, mannitol, sorbitol, etc.); vitrification technique and the preparation of vitrification solutions (PVS1, PVS2, PVS3, etc.); desiccation technique (silica gel and laminar flow cabinet); synthetic seed production (encapsulation); encapsulation dehydration; desiccation encapsulation; droplet method.
Course Precondition
no
Resources
Notes and presentations prepared within the scope of the course content.
Notes
Plant Cryopreservation Carla Benelli (Ed.)
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Have knowledge about plant genetic resources and conservation. |
| LO02 | Knows and applies different Croyopreservation methods. |
| LO03 | Knows the issues of synthetic seeds and their use, comprehends the biotechnological importance. |
| LO04 | Carrying out a study individually that requires professional knowledge in Cryopreservation |
| LO05 | Entering database related to horticulture, utilizing those sources and self-updating. |
| LO06 | Evaluating the knowledge and skills in horticulture by criticism and directing their own learning process |
| LO07 | Presenting their studies, developments of their expertise topic and research results by orally, written and taking advantage of visual equipments |
| LO08 | Obtaining data by scientific methods in order to solve a problem related to horticulture, establishing and interpreting the data by considering the scientific and ethical values |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | To be able to develop and deepen knowledge acquired in horticultural field based on undergraduate level competencies | 1 |
| PLO02 | Bilgi - Kuramsal, Olgusal | To be able to understand the interaction between horticultural field and related disciplines | 2 |
| PLO03 | Bilgi - Kuramsal, Olgusal | To be able to combine and interpret the theoretical and practical knowledge related to horticultural plants with the knowledge, data and findings obtained from different disciplines, to be able to create new knowledge and theories by synthesizing them by using the theoretical and practical knowledge related to horticultural plants in the field of expertise and supporting the current developments with quantitative and qualitative | 2 |
| PLO04 | Bilgi - Kuramsal, Olgusal | Solving problems by using research methods and solving cause and effect relationships | 2 |
| PLO05 | Bilgi - Kuramsal, Olgusal | To be able to conduct a study requiring expertise on horticultural plants independently | 2 |
| PLO06 | Bilgi - Kuramsal, Olgusal | To develop an analytical approach for solving unpredictable complex problems encountered in horticultural applications, to design the research process, to produce solutions by taking responsibility and to evaluate and defend the results obtained | 1 |
| PLO07 | Bilgi - Kuramsal, Olgusal | To lead the horticultural plants in an environment that requires solving problems | |
| PLO08 | Beceriler - Bilişsel, Uygulamalı | Access to resources related to garden plants, benefiting from these resources and self-renewal | 2 |
| PLO09 | Beceriler - Bilişsel, Uygulamalı | To be able to evaluate the knowledge and skills acquired in the field of horticulture with a critical approach and to direct the learning process | 2 |
| PLO10 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | To be able to transfer his / her studies, developments in his / her field of expertise and research results using oral, written and visual tools | 3 |
| PLO11 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | To be able to use advanced computer software and information and communication technologies as necessary in relation to horticultural plants | |
| PLO12 | Yetkinlikler - Öğrenme Yetkinliği | To be able to collect data with scientific methods for solving problems related to horticultural plants, to be able to control and interpret the collected data by taking into consideration the social, scientific and ethical values. | |
| PLO13 | Yetkinlikler - Öğrenme Yetkinliği | To be able to use assimilated information in the field of horticulture in interdisciplinary studies, explain it, transfer it to others, examine the results from a critical perspective |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Plant Genetic Resources and consevation, The historical development of cryopreservation techniques | Reading the related lecture notes | |
| 2 | Slow cooling techniques | Reading the related lecture notes | |
| 3 | Rapid cooling techniques | Reading the related lecture notes | |
| 4 | Cold hardening | Reading the related lecture notes | |
| 5 | Cryoprotectans | Reading the related lecture notes | |
| 6 | Vitrification technique and preparation of solutions | Reading the related lecture notes | |
| 7 | Desiccation technique | Reading the related lecture notes | |
| 8 | Mid-Term Exam | Reading the related lecture notes | |
| 9 | Production of synthetic seeds | Reading the related lecture notes | |
| 10 | Encapsulation dehydration | Reading the related lecture notes | |
| 11 | Encapsulation desiccation | Reading the related lecture notes | |
| 12 | Encapsulation vitrification | Reading the related lecture notes | |
| 13 | Cryopreservation of embriyogenic culture | Reading the related lecture notes | |
| 14 | Cryopreservation of Bryophytes and fern | Reading the related lecture notes | |
| 15 | Droplet method | Reading the related lecture notes | |
| 16 | Term Exams | Reading the related lecture notes | |
| 17 | Term Exams | Reading the related lecture notes |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 4 | 56 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 1 | 2 | 2 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 12 | 12 |
| Final Exam | 1 | 28 | 28 |
| Total Workload (Hour) | 154 | ||
| Total Workload / 25 (h) | 6,16 | ||
| ECTS | 6 ECTS | ||