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Information
| Code | BB595 |
| Name | Laboratory Technics of Plant Biotechnology |
| Term | 2022-2023 Academic Year |
| Term | Spring |
| Duration (T+A) | 2-2 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. YILDIZ KAÇAR |
| Course Instructor |
1 |
Course Goal / Objective
The aim of this course is to examine the main techniques applied in plant biotechnology laboratory.
Course Content
The contents of this course include the definition and application of plant biotechnology, solution preparation, microscope, centrifuge, use of PHmeter in biotechnology, plant tissue cultures, the preparation of liquid-solid LB medium, creation of a single colony of bacteria, Agrobacterium tumefaciens-mediated gene transfer, DNA isolation and quantification, PCR applications, agarose gel electrophoresis, polyacrylamide gel electrophoresis, DNA sequence analysis.
Course Precondition
No
Resources
Notes and presentations prepared within the scope of the course content.
Notes
At the Bench a Laboratory navigator KAthy Barker Plant Biotechnology: Lab Course Robert J. Lebowitz
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Developing and improving the plant biotechnology knowledge to professional level based on the bachelors degree qualifications |
| LO02 | Understanding the plant biotechnology and the interaction between the related disciplines |
| LO03 | Solving problems by research methods and establishing cause-effect relationship |
| LO04 | Carrying out a study individually that requires professional knowledge in Horticulture |
| LO05 | Knows Plant Biotechnology and Applications |
| LO06 | Using Plant Tissue Culture and Molecular techniques in the Plant breeding |
| LO07 | Entering database related to plant biotechnology, utilizing those sources and self-updating |
| LO08 | Evaluation of the basic techniques used in plant biotechnology |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | To be able to develop and deepen knowledge acquired in horticultural field based on undergraduate level competencies | 1 |
| PLO02 | Bilgi - Kuramsal, Olgusal | To be able to understand the interaction between horticultural field and related disciplines | 2 |
| PLO03 | Bilgi - Kuramsal, Olgusal | To be able to combine and interpret the theoretical and practical knowledge related to horticultural plants with the knowledge, data and findings obtained from different disciplines, to be able to create new knowledge and theories by synthesizing them by using the theoretical and practical knowledge related to horticultural plants in the field of expertise and supporting the current developments with quantitative and qualitative | 1 |
| PLO04 | Bilgi - Kuramsal, Olgusal | Solving problems by using research methods and solving cause and effect relationships | 2 |
| PLO05 | Bilgi - Kuramsal, Olgusal | To be able to conduct a study requiring expertise on horticultural plants independently | 2 |
| PLO06 | Bilgi - Kuramsal, Olgusal | To develop an analytical approach for solving unpredictable complex problems encountered in horticultural applications, to design the research process, to produce solutions by taking responsibility and to evaluate and defend the results obtained | 2 |
| PLO07 | Bilgi - Kuramsal, Olgusal | To lead the horticultural plants in an environment that requires solving problems | |
| PLO08 | Beceriler - Bilişsel, Uygulamalı | Access to resources related to garden plants, benefiting from these resources and self-renewal | 2 |
| PLO09 | Beceriler - Bilişsel, Uygulamalı | To be able to evaluate the knowledge and skills acquired in the field of horticulture with a critical approach and to direct the learning process | 2 |
| PLO10 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | To be able to transfer his / her studies, developments in his / her field of expertise and research results using oral, written and visual tools | 3 |
| PLO11 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | To be able to use advanced computer software and information and communication technologies as necessary in relation to horticultural plants | |
| PLO12 | Yetkinlikler - Öğrenme Yetkinliği | To be able to collect data with scientific methods for solving problems related to horticultural plants, to be able to control and interpret the collected data by taking into consideration the social, scientific and ethical values. | 2 |
| PLO13 | Yetkinlikler - Öğrenme Yetkinliği | To be able to use assimilated information in the field of horticulture in interdisciplinary studies, explain it, transfer it to others, examine the results from a critical perspective | 3 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Definition and application of biotechnology of plant biotechnology | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım, Tartışma, Gösteri, Deney / Laboratuvar |
| 2 | Preparation of the solutions | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım, Tartışma, Gösteri, Deney / Laboratuvar |
| 3 | Use of microscope, centrifuge, PH meter in biotechnology | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım, Tartışma, Gösteri, Deney / Laboratuvar |
| 4 | Conditions laboratory of plant tissue cultures and sterilization | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım, Tartışma, Gösteri, Deney / Laboratuvar |
| 5 | Plant tissue cultures | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım, Tartışma, Gösteri, Deney / Laboratuvar |
| 6 | Preparation of liquid-solid LB medium, creation of a single colony of bacteria | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım, Tartışma, Gösteri, Deney / Laboratuvar |
| 7 | Agrobacterium tumefaciens mediated gene transfer | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım, Tartışma, Gösteri, Deney / Laboratuvar |
| 8 | Mid-Term Exam | Preparation of the exam | Ölçme Yöntemleri: Yazılı Sınav |
| 9 | DNA isolation and determination of quality and quantity | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım, Tartışma, Gösteri, Deney / Laboratuvar |
| 10 | RNA and mRNA isolation, cDNA synthesis and determination of the quality-quantity | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım, Tartışma, Gösteri, Deney / Laboratuvar |
| 11 | application of PCR | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım, Tartışma, Gösteri, Deney / Laboratuvar |
| 12 | Agarose gel electrophoresis | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım, Tartışma, Gösteri, Deney / Laboratuvar |
| 13 | Polyacyrilamide gelelectrophoresis | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım, Tartışma, Gösteri, Deney / Laboratuvar |
| 14 | DNA bands imaging methods in polyacrylamide gel | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım, Tartışma, Gösteri, Deney / Laboratuvar |
| 15 | DNA sequencing | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım, Tartışma, Gösteri, Deney / Laboratuvar |
| 16 | Term Exams | Preparation of the exam | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | Preparation of the exam | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 4 | 56 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 1 | 2 | 2 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 12 | 12 |
| Final Exam | 1 | 28 | 28 |
| Total Workload (Hour) | 154 | ||
| Total Workload / 25 (h) | 6,16 | ||
| ECTS | 6 ECTS | ||