TÜRKÇE
ENGLISH
Information
| Code | BB030 |
| Name | Using of Molecular Techniques in Hoticultural Plants |
| Term | 2022-2023 Academic Year |
| Semester | . Semester |
| Duration (T+A) | 4-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 4 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. YILDIZ KAÇAR |
Course Goal / Objective
Information about DNA,RNA and PCR, types of molecular markers, advantages / disadvantages and usage areas of horticulture breeding
Course Content
Information about DNA, RNA and PCR, solution preparation, types of molecular markers (RAPD, SSR, ISSR etc.), advantages / disadvantages of molecular markers and their use in plant breeding
Course Precondition
No
Resources
Notes and presentations prepared within the scope of the course content.
Notes
Molecular Techniques in Crop Improvement Editors: S. Mohan Jain, D. S. Brar, B. S. Ahloowalia
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Knows DNA-RNA structure, DNA-RNA isolation in plants. |
| LO02 | Have knowledge about PCR technologies and Electrophoresis systems and can perform their applications. |
| LO03 | Comprehends molecular marker technology and interprets the possibilities of use in plants. |
| LO04 | Learns the techniques used in plant molecular biology. |
| LO05 | Carrying out a study individually that requires professional knowledge in Horticulture and Molecular Biology |
| LO06 | Using software related to horticulture and molecular markers sufficiently and using information and communication technologies in advanced level |
| LO07 | To be able to understand the concepts of ethics and biosafety in horticulture molecular breeding |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | To be able to develop and deepen knowledge acquired in horticultural field based on undergraduate level competencies | 1 |
| PLO02 | Bilgi - Kuramsal, Olgusal | To be able to understand the interaction between horticultural field and related disciplines | 2 |
| PLO03 | Bilgi - Kuramsal, Olgusal | To be able to combine and interpret the theoretical and practical knowledge related to horticultural plants with the knowledge, data and findings obtained from different disciplines, to be able to create new knowledge and theories by synthesizing them by using the theoretical and practical knowledge related to horticultural plants in the field of expertise and supporting the current developments with quantitative and qualitative | |
| PLO04 | Bilgi - Kuramsal, Olgusal | Solving problems by using research methods and solving cause and effect relationships | 1 |
| PLO05 | Bilgi - Kuramsal, Olgusal | To be able to conduct a study requiring expertise on horticultural plants independently | |
| PLO06 | Bilgi - Kuramsal, Olgusal | To develop an analytical approach for solving unpredictable complex problems encountered in horticultural applications, to design the research process, to produce solutions by taking responsibility and to evaluate and defend the results obtained | |
| PLO07 | Bilgi - Kuramsal, Olgusal | To lead the horticultural plants in an environment that requires solving problems | |
| PLO08 | Beceriler - Bilişsel, Uygulamalı | Access to resources related to garden plants, benefiting from these resources and self-renewal | 2 |
| PLO09 | Beceriler - Bilişsel, Uygulamalı | To be able to evaluate the knowledge and skills acquired in the field of horticulture with a critical approach and to direct the learning process | 1 |
| PLO10 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | To be able to transfer his / her studies, developments in his / her field of expertise and research results using oral, written and visual tools | 3 |
| PLO11 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | To be able to use advanced computer software and information and communication technologies as necessary in relation to horticultural plants | |
| PLO12 | Yetkinlikler - Öğrenme Yetkinliği | To be able to collect data with scientific methods for solving problems related to horticultural plants, to be able to control and interpret the collected data by taking into consideration the social, scientific and ethical values. | 2 |
| PLO13 | Yetkinlikler - Öğrenme Yetkinliği | To be able to use assimilated information in the field of horticulture in interdisciplinary studies, explain it, transfer it to others, examine the results from a critical perspective | 3 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | DNA, RNA theoretical expression, DNA isolation in plants | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım |
| 2 | Introduction of molecular genetics laboratory, Laboratory Practice | Reading the related lecture notes | Öğretim Yöntemleri: Deney / Laboratuvar |
| 3 | Polymerase Chain Reactions, Electrophoresis Systems | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım |
| 4 | Laboratory Practice: PCR optimization - preparation of PCR and determination of cycle conditions | Reading the related lecture notes | Öğretim Yöntemleri: Deney / Laboratuvar |
| 5 | Biochemical markers and their use in plant breeding, marker systems based on hybridization and their use in breeding (RFLP) | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım |
| 6 | Laboratory Practice: Preparation of agarose and polyacrylamide gel | Reading the related lecture notes | Öğretim Yöntemleri: Deney / Laboratuvar |
| 7 | Molecular Markers (RAPD, ISSR, SRAP) | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım |
| 8 | Mid-Term Exam | Preparation for the exam | Ölçme Yöntemleri: Yazılı Sınav |
| 9 | Laboratory Practice: RAPD, ISSR and SRAP | Reading the related lecture notes | Öğretim Yöntemleri: Deney / Laboratuvar |
| 10 | 38/5000 Molecular Markers (SSR, AFLP, SSCP) | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım |
| 11 | Laboratory Practice: SSR, AFLP and SSCP | Reading the related lecture notes | Öğretim Yöntemleri: Deney / Laboratuvar |
| 12 | 36/5000 Genetic Mapping in Plants and MAS | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım |
| 13 | Laboratory Practice: Genetic Maps | Reading the related lecture notes | Öğretim Yöntemleri: Deney / Laboratuvar |
| 14 | Methods for Determining Gene Expression in Plants -cDNA AFLP, Microarray, RNA seq, Real Time PCR | Reading the related lecture notes | Öğretim Yöntemleri: Anlatım |
| 15 | Laboratory Practice: Real Time PCR | Reading the related lecture notes | Öğretim Yöntemleri: Deney / Laboratuvar |
| 16 | Term Exams | Preparation for the exam | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | Preparation for the exam | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 4 | 56 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 12 | 12 |
| Final Exam | 1 | 28 | 28 |
| Total Workload (Hour) | 152 | ||
| Total Workload / 25 (h) | 6,08 | ||
| ECTS | 6 ECTS | ||