TÜRKÇE
ENGLISH
Information
| Code | BT553 |
| Name | Analysis of transgenic plants and propagation materials in Genetically modified organisms |
| Term | 2022-2023 Academic Year |
| Semester | . Semester |
| Duration (T+A) | 3-2 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 4 National Credit |
| Teaching Language | Türkçe |
| Level | Doktora Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. MUKADDES KAYIM |
Course Goal / Objective
Giving information about genetically modified plant production techniques and their contents in the world. To teach how to do analysis of such plants and production materials.
Course Content
Trangenic plant production methods will be explained in detail. The characteristics of commercial transgenic products will be discussed, the analysis methods will be explained in detail and will be shown in practice.
Course Precondition
No pre requirement
Resources
Principles of Gene manipulation (R.W. Old and S.B. Primrose, 1992), Technology transfer of plant biotechnology (Petter M. Gresshoff, NewYork, 1994), Transgenic plants research (Keith Lindsey 1997)
Notes
Some published articles
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Gains general information about genetically modified organisms. Learn gene transfer techniques |
| LO02 | Learn about the use of transgenic plants in agriculture |
| LO03 | Develops an opinion on the positive and negative effects of transgenic plants on the environment |
| LO04 | Learn molecular, histochemical assay and tissue culture methods for trangenic plant detection. |
| LO05 | Learns which methods and genes are used in GMO analysis in the world and inTurkish Standards Institute. |
| LO06 | Learns vectors used in transgenic plant production and how these vectors are used in GMO analysis |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Develop and deepen the current and advanced knowledge in the field with original thought and / or research at the level of expertise based on the qualifications of the graduate and reach the original definitions that will bring innovation to the field. | 5 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Understands the interdisciplinary interaction of the field; it achieves original results using knowledge requiring expertise in analysis, synthesis and evaluation of new and complex ideas. | |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | Evaluates, uses and transfers new information in the field with a systematic approach. | |
| PLO04 | Beceriler - Bilişsel, Uygulamalı | Makes critical analysis, synthesis and evaluation of new and complex thoughts. | |
| PLO05 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Makes leadership in environments that require the analysis of original and interdisciplinary problems. | |
| PLO06 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | It extends the limits of knowledge in its field by publishing at least one scientific article in its field in national and / or international refereed journals and / or producing or interpreting an original work. | 1 |
| PLO07 | Yetkinlikler - Öğrenme Yetkinliği | Develops new ideas and methods related to the field by using high level mental processes such as creative and critical thinking, problem solving and decision making. | 3 |
| PLO08 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | He / she defends his / her original opinions in discussing the subjects in his / her field and establishes an effective communication showing his / her competence in the field. | 4 |
| PLO09 | Yetkinlikler - Alana Özgü Yetkinlik | Conducts scientific research in national and international scientific research groups. | 2 |
| PLO10 | Yetkinlikler - Alana Özgü Yetkinlik | Establishes functional interaction by using strategic decision making processes in solving problems encountered in the field. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Introduction to Genetic transformation | Related articles, books and lecture notes | Öğretim Yöntemleri: Anlatım, Gösteri |
| 2 | Basic principles of Agrobacterium-mediated gene transfer | Related articles, books and lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Gösteri |
| 3 | Gene transfer to plant tissues and protoplasts | Related articles, books and lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Gösteri |
| 4 | Bacterial and vectoral plasmids | Related articles, books and lecture notes | Öğretim Yöntemleri: Anlatım, Gösteri |
| 5 | Gene cloning | Related articles, books and lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Gösteri |
| 6 | Properties of marker genes | Related articles, books and lecture notes | Öğretim Yöntemleri: Anlatım, Gösteri |
| 7 | Analysis of Kanamycin and Hygromycin antibiotics | Related articles, books and lecture notes | Öğretim Yöntemleri: Anlatım, Gösteri, Deney / Laboratuvar |
| 8 | Mid-Term Exam | Written exam | Ölçme Yöntemleri: Ödev |
| 9 | Bacterial and viral Promoter genes | Related articles, books and lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Gösteri |
| 10 | Cauliflower Mosaic virus 35 S promoter | Related articles, books and lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Gösteri, Deney / Laboratuvar |
| 11 | Detection of GMO gene copy numbers with Southern blot | Related articles, books and lecture notes | Öğretim Yöntemleri: Anlatım, Gösteri |
| 12 | GMO analysis genes used by Turkish Standards Institute | Related articles, books and lecture notes | Öğretim Yöntemleri: Anlatım, Tartışma |
| 13 | Resistance genes used against bacteria | Related articles | Öğretim Yöntemleri: Soru-Cevap, Tartışma |
| 14 | What are resistance genes against fungi | Related articles, books and lecture notes | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 15 | Green flourecent gene analysis | Related articles | Öğretim Yöntemleri: Tartışma, Gösteri |
| 16 | Term Exams | Written exam | Ölçme Yöntemleri: Yazılı Sınav, Sözlü Sınav, Ödev, Performans Değerlendirmesi |
| 17 | Term Exams | Written exam | Ölçme Yöntemleri: Yazılı Sınav, Ödev |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 5 | 70 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 5 | 70 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 5 | 5 |
| Final Exam | 1 | 15 | 15 |
| Total Workload (Hour) | 160 | ||
| Total Workload / 25 (h) | 6,40 | ||
| ECTS | 6 ECTS | ||