TÜRKÇE
ENGLISH
Information
| Code | TB607 |
| Name | Laboratory Techniques in Biotechnology |
| Term | 2022-2023 Academic Year |
| Semester | . Semester |
| Duration (T+A) | 2-2 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator |
Course Goal / Objective
To teach the concept of biotechnology, plant biotechnology and in vitro culture, and to give some information about methodologies used in plant biotechnology
Course Content
This course contains the concept of biotechnology, plant biotechnology and in vitro culture, introduction to plant biotechnology laboratory, plant culture medium used in in vitro culture, preparing stock solution for macro and microelements, preparing stock solution for plant hormone and vitamins, sterilization for glass and metal materials, preparing plant culture medium, principles of working in the sterile cabin, sterilization of plant materials, embryo culture, anther culture, DNA isolation and PCR analysis
Course Precondition
Non
Resources
Hatipoğlu, R. 1993. Lecture notes of laboratory techniques in biotechnology
Notes
Reinert, J. and Yeoman, M.M. 1982. Plant Cell and Tissue Culture, A Laboratory Manual. Springer Verlag, Heidelberg.
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Learn the concept of biotechnology, plant biotechnology and invitro culture |
| LO02 | Learn how to use the equipment of laboratory |
| LO03 | Learn steril work, preparation of plant tissues medium, some techniques for in vitro, isolation of DNA and PCR analysis |
| LO04 | Learn sterile work and safety rules |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Graduates become a specialist on the field crops area by improving their skills. | 4 |
| PLO02 | Bilgi - Kuramsal, Olgusal | They comprehend interdisciplinary interaction in his specialization area. | |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | They can follow the latest developments in field crops area and get access to the knowledge source, gather this knowledge, reach new knowledge and synthesize by evaluating available studies and follow the innovations in his field. | 4 |
| PLO04 | Beceriler - Bilişsel, Uygulamalı | They can synthesize and interpret interdisciplinary knowledge by using theoretical and practical skills at a specialist level in field crops area. | |
| PLO05 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | They find solutions for problems related to field crops by using research methods and establish cause effect relationships. | |
| PLO06 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | They incorporate their knowledge on his special area by combining their knowledge with those from the other scientific areas and produce new knowledge and also solve the problems by using scientific research methods. | |
| PLO07 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | They determine a problem independently in field crops subject, provide solutions, evaluate the results and implement whenever required. | |
| PLO08 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | They are qualified to solve a problem in their field. | |
| PLO09 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | They are equipped with analytical and critical thinking ability to direct their learning and to conduct advanced studies in their fields independently. | |
| PLO10 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | They transfer current developments and their own studies in the field crops area systematically to the his working group and to different groups from other study fields orally or visually by supporting them with qualitative and quantitive data. | |
| PLO11 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | They study and improve the social relationships and standards leading these relationship by a critical point of view and take action when needed. | |
| PLO12 | Yetkinlikler - Alana Özgü Yetkinlik | They are able to understand and translate an article written in a foreign language. | |
| PLO13 | Yetkinlikler - Alana Özgü Yetkinlik | They develop policy, strategy and experimental plans related to field crops area and evaluate obtained results within the framework of quality processes. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Explaining the concept of biotechnology, plant biotechnology and invitro culture | Pre reading | Öğretim Yöntemleri: Anlatım |
| 2 | Introduction to plant biotechnology laboratory | Pre reading | Öğretim Yöntemleri: Anlatım |
| 3 | Plant culture medium used in in vitro culture | Pre reading | Öğretim Yöntemleri: Anlatım |
| 4 | Preparing stock solution for macro and microelements | Pre reading | Öğretim Yöntemleri: Anlatım |
| 5 | Preparing stock solution for plant hormone and vitamins | Pre reading | Öğretim Yöntemleri: Anlatım |
| 6 | Sterilization principles for glass and metal materials | Pre reading | Öğretim Yöntemleri: Anlatım |
| 7 | Preparing plant culture medium | Pre reading | Öğretim Yöntemleri: Anlatım |
| 8 | Mid-Term Exam | Preparation of homework | Ölçme Yöntemleri: Ödev |
| 9 | Principles of working the sterile cabin | Pre reading | Öğretim Yöntemleri: Anlatım |
| 10 | Sterilization of plant materials and an assignment will be given on this topic | Pre reading | Öğretim Yöntemleri: Anlatım |
| 11 | Embryo culture | Pre reading | Öğretim Yöntemleri: Anlatım |
| 12 | Anther culture | Pre reading | Öğretim Yöntemleri: Anlatım |
| 13 | DNA isolation | Pre reading | Öğretim Yöntemleri: Anlatım |
| 14 | PCR analysis | Pre reading | Öğretim Yöntemleri: Anlatım |
| 15 | Evaluation and interpretation of PCR analysis | Pre reading | Öğretim Yöntemleri: Anlatım |
| 16 | Term Exams | Preparation for exam | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | Preparation for exam | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 4 | 56 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 1 | 2 | 2 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 12 | 12 |
| Final Exam | 1 | 28 | 28 |
| Total Workload (Hour) | 154 | ||
| Total Workload / 25 (h) | 6,16 | ||
| ECTS | 6 ECTS | ||