TB607 Laboratory Techniques in Biotechnology

6 ECTS - 2-2 Duration (T+A)- . Semester- 3 National Credit

Information

Code TB607
Name Laboratory Techniques in Biotechnology
Term 2022-2023 Academic Year
Semester . Semester
Duration (T+A) 2-2 (T-A) (17 Week)
ECTS 6 ECTS
National Credit 3 National Credit
Teaching Language Türkçe
Level Yüksek Lisans Dersi
Type Normal
Mode of study Yüz Yüze Öğretim
Catalog Information Coordinator


Course Goal / Objective

To teach the concept of biotechnology, plant biotechnology and in vitro culture, and to give some information about methodologies used in plant biotechnology

Course Content

This course contains the concept of biotechnology, plant biotechnology and in vitro culture, introduction to plant biotechnology laboratory, plant culture medium used in in vitro culture, preparing stock solution for macro and microelements, preparing stock solution for plant hormone and vitamins, sterilization for glass and metal materials, preparing plant culture medium, principles of working in the sterile cabin, sterilization of plant materials, embryo culture, anther culture, DNA isolation and PCR analysis

Course Precondition

Non

Resources

Hatipoğlu, R. 1993. Lecture notes of laboratory techniques in biotechnology

Notes

Reinert, J. and Yeoman, M.M. 1982. Plant Cell and Tissue Culture, A Laboratory Manual. Springer Verlag, Heidelberg.


Course Learning Outcomes

Order Course Learning Outcomes
LO01 Learn the concept of biotechnology, plant biotechnology and invitro culture
LO02 Learn how to use the equipment of laboratory
LO03 Learn steril work, preparation of plant tissues medium, some techniques for in vitro, isolation of DNA and PCR analysis
LO04 Learn sterile work and safety rules


Relation with Program Learning Outcome

Order Type Program Learning Outcomes Level
PLO01 Bilgi - Kuramsal, Olgusal Graduates become a specialist on the field crops area by improving their skills. 4
PLO02 Bilgi - Kuramsal, Olgusal They comprehend interdisciplinary interaction in his specialization area.
PLO03 Beceriler - Bilişsel, Uygulamalı They can follow the latest developments in field crops area and get access to the knowledge source, gather this knowledge, reach new knowledge and synthesize by evaluating available studies and follow the innovations in his field. 4
PLO04 Beceriler - Bilişsel, Uygulamalı They can synthesize and interpret interdisciplinary knowledge by using theoretical and practical skills at a specialist level in field crops area.
PLO05 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği They find solutions for problems related to field crops by using research methods and establish cause effect relationships.
PLO06 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği They incorporate their knowledge on his special area by combining their knowledge with those from the other scientific areas and produce new knowledge and also solve the problems by using scientific research methods.
PLO07 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği They determine a problem independently in field crops subject, provide solutions, evaluate the results and implement whenever required.
PLO08 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği They are qualified to solve a problem in their field.
PLO09 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği They are equipped with analytical and critical thinking ability to direct their learning and to conduct advanced studies in their fields independently.
PLO10 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği They transfer current developments and their own studies in the field crops area systematically to the his working group and to different groups from other study fields orally or visually by supporting them with qualitative and quantitive data.
PLO11 Yetkinlikler - İletişim ve Sosyal Yetkinlik They study and improve the social relationships and standards leading these relationship by a critical point of view and take action when needed.
PLO12 Yetkinlikler - Alana Özgü Yetkinlik They are able to understand and translate an article written in a foreign language.
PLO13 Yetkinlikler - Alana Özgü Yetkinlik They develop policy, strategy and experimental plans related to field crops area and evaluate obtained results within the framework of quality processes.


Week Plan

Week Topic Preparation Methods
1 Explaining the concept of biotechnology, plant biotechnology and invitro culture Pre reading Öğretim Yöntemleri:
Anlatım
2 Introduction to plant biotechnology laboratory Pre reading Öğretim Yöntemleri:
Anlatım
3 Plant culture medium used in in vitro culture Pre reading Öğretim Yöntemleri:
Anlatım
4 Preparing stock solution for macro and microelements Pre reading Öğretim Yöntemleri:
Anlatım
5 Preparing stock solution for plant hormone and vitamins Pre reading Öğretim Yöntemleri:
Anlatım
6 Sterilization principles for glass and metal materials Pre reading Öğretim Yöntemleri:
Anlatım
7 Preparing plant culture medium Pre reading Öğretim Yöntemleri:
Anlatım
8 Mid-Term Exam Preparation of homework Ölçme Yöntemleri:
Ödev
9 Principles of working the sterile cabin Pre reading Öğretim Yöntemleri:
Anlatım
10 Sterilization of plant materials and an assignment will be given on this topic Pre reading Öğretim Yöntemleri:
Anlatım
11 Embryo culture Pre reading Öğretim Yöntemleri:
Anlatım
12 Anther culture Pre reading Öğretim Yöntemleri:
Anlatım
13 DNA isolation Pre reading Öğretim Yöntemleri:
Anlatım
14 PCR analysis Pre reading Öğretim Yöntemleri:
Anlatım
15 Evaluation and interpretation of PCR analysis Pre reading Öğretim Yöntemleri:
Anlatım
16 Term Exams Preparation for exam Ölçme Yöntemleri:
Yazılı Sınav
17 Term Exams Preparation for exam Ölçme Yöntemleri:
Yazılı Sınav


Student Workload - ECTS

Works Number Time (Hour) Workload (Hour)
Course Related Works
Class Time (Exam weeks are excluded) 14 4 56
Out of Class Study (Preliminary Work, Practice) 14 4 56
Assesment Related Works
Homeworks, Projects, Others 1 2 2
Mid-term Exams (Written, Oral, etc.) 1 12 12
Final Exam 1 28 28
Total Workload (Hour) 154
Total Workload / 25 (h) 6,16
ECTS 6 ECTS

Update Time: 20.11.2022 03:43