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Information
| Code | HİS629 |
| Name | Cell culture Technique Practice |
| Term | 2022-2023 Academic Year |
| Term | Spring |
| Duration (T+A) | 0-4 (T-A) (17 Week) |
| ECTS | 4 ECTS |
| National Credit | 2 National Credit |
| Teaching Language | Türkçe |
| Level | Doktora Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Doç. Dr. YURDUN KUYUCU |
| Course Instructor |
1 |
Course Goal / Objective
The aim of this course is to learn and practice the cell culture techniques.
Course Content
Regulation of cell culture laboratory sterilization techniques, preparation of the solution used in the culture, preparation of tissues and primary cultures applications, passaged cell freezing and thawing, immunohistochemical applications the culture medium, photographing cells examined with an inverted microscope.
Course Precondition
Stem Cell Biology
Resources
Kök Hücre. Alp Can, Akademisyen Kitabevi. Histolojik Boyama Teknikleri. Prof. Dr. Ramazan Demir, Palme Yayınevi.
Notes
Kök Hücre. Alp Can, Akademisyen Kitabevi. Histolojik Boyama Teknikleri. Prof. Dr. Ramazan Demir, Palme Yayınevi.
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Getting knowledge about general regulation of cell culture laboratory and the tools used in the laboratory. |
| LO02 | Getting knowledge about sterilization techniques and safety. |
| LO03 | Prepares serum, medium and other solutions used in the culture. |
| LO04 | Obtains tissues for primary culture and produces cells in the culture medium. |
| LO05 | Applies passages, freezing and thawing techniques. |
| LO06 | Applies cell viability tests, cell count and immunohistochemical methods. |
| LO07 | Examines cells and takes photographs by the inverted microscope and interprets them. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Have knowledge about structure and function of tissue and organ systems. | |
| PLO02 | Bilgi - Kuramsal, Olgusal | Have knowledge about implantation biology and IVF techniques. | |
| PLO03 | Bilgi - Kuramsal, Olgusal | Gains knowledge of the normal development of tissue and organ systems, developmental disorders and mechanisms. | |
| PLO04 | Bilgi - Kuramsal, Olgusal | Knows advanced light and electron microscopic tissue preparation, histochemical, immunohistochemistry and cell culture techniques according to the study area. | 5 |
| PLO05 | Bilgi - Kuramsal, Olgusal | Has knowledge about the basic properties of stem cells. | 5 |
| PLO06 | Bilgi - Kuramsal, Olgusal | Has advanced knowledge about technological tools, devices and tools used in histological studies | 5 |
| PLO07 | Bilgi - Kuramsal, Olgusal | Knows the importance of ethical rules and ethical principles. | |
| PLO08 | Bilgi - Kuramsal, Olgusal | Has knowledge about planning of experimental studies and statistical methods. | |
| PLO09 | Bilgi - Kuramsal, Olgusal | Prepares and examines the tissues using electron microscopic, immunohistochemistry and special staining techniques. | 5 |
| PLO10 | Bilgi - Kuramsal, Olgusal | Usestechnological tools, devices and equipment according to the level. | 5 |
| PLO11 | Bilgi - Kuramsal, Olgusal | Prepares experimental studies as a project. | |
| PLO12 | Bilgi - Kuramsal, Olgusal | Presents the results of research in scientific meetings and publishes them in refereed journals. | |
| PLO13 | Bilgi - Kuramsal, Olgusal | Makes critical analysis, synthesis and evaluation related to the field. | 5 |
| PLO14 | Bilgi - Kuramsal, Olgusal | Carries out studies individually or as a team member and leads the necessary environments. | |
| PLO15 | Bilgi - Kuramsal, Olgusal | Bilimsel bir çalışmayı bağımsız olarak planlayıp, gerçekleştirerek istatistiksel analizlerini yapar. | |
| PLO16 | Beceriler - Bilişsel, Uygulamalı | Uses the principles of professional development and lifelong learning in their studies. | |
| PLO17 | Beceriler - Bilişsel, Uygulamalı | Uses critical thinking, decision making and evidence-based medicine applications in their research. | |
| PLO18 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | By communicating effectively, discusses the issues related to the field and defends their views. | |
| PLO19 | Yetkinlikler - Öğrenme Yetkinliği | Works with relevant disciplines in the process of selecting, applying and interpreting appropriate methods for studies and establish functional communication. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Regulation and safety of cell culture laboratory | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 2 | Usage of laminar flow cabinet, incubator and autoclaves | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 3 | Preparation of the solution, medium, serum etc. used in the culture medium | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 4 | The cultivation of obtained dermal fibroblasts with the culture dish | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 5 | Examination of confluent cultures under the microscope and passages them | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 6 | Preparation of cells to be frozen | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 7 | Cryopreservation and thawing of the cells | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 8 | Mid-Term Exam | Ölçme Yöntemleri: Yazılı Sınav |
|
| 9 | Immunohistochemistry applications in the culture medium | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 10 | Transfection techniques in the cell culture | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 11 | Cell viability and cell count applications | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 12 | Microscope types used in the cell culture | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 13 | Invert microscopic analysis of cells grown in culture and taking photography | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 14 | Contamination problems and their solutions in the cell culture laboratory | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 15 | Sterilization techniques | Self-study, reading recommended references | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 16 | Term Exams | Ölçme Yöntemleri: Yazılı Sınav |
|
| 17 | Term Exams | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 2 | 28 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 8 | 8 |
| Final Exam | 1 | 8 | 8 |
| Total Workload (Hour) | 100 | ||
| Total Workload / 25 (h) | 4,00 | ||
| ECTS | 4 ECTS | ||