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Information
| Code | HİS605 |
| Name | Basic cell culture techniques and examination methods |
| Term | 2022-2023 Academic Year |
| Semester | . Semester |
| Duration (T+A) | 3-0 (T-A) (17 Week) |
| ECTS | 4 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Doktora Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Doç. Dr. LEMAN SENCAR GÜNDOĞDU |
Course Goal / Objective
The aim of this course is to get information about basic cell culture techniques and usage areas.
Course Content
Cell culture terminology, adherent and suspansion cultures, primary and secondary cultures, media, serum, cell culture usage areas, cell viability tests, immunocytochemical techniques.
Course Precondition
None
Resources
Michael H. Ross, Wojciech Pawlina Histoloji Lecture and Atlas
Notes
Lecture slides and notes
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | 1. Knows cell culture terminology. |
| LO02 | 2. Getting information about cell culture techniques, serum and media. |
| LO03 | 3. Knows adherent and suspansion cultures. Gets information about primary and secondary cultures. |
| LO04 | 4. Knows usage areas of cell culture. |
| LO05 | 5. Getting information about cell viability tests and immunocytochemistry methods. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Have knowledge about structure and function of tissue and organ systems. | 1 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Have knowledge about implantation biology and IVF techniques. | 1 |
| PLO03 | Bilgi - Kuramsal, Olgusal | Gains knowledge of the normal development of tissue and organ systems, developmental disorders and mechanisms. | 2 |
| PLO04 | Bilgi - Kuramsal, Olgusal | Knows advanced light and electron microscopic tissue preparation, histochemical, immunohistochemistry and cell culture techniques according to the study area. | |
| PLO05 | Bilgi - Kuramsal, Olgusal | Has knowledge about the basic properties of stem cells. | 5 |
| PLO06 | Bilgi - Kuramsal, Olgusal | Has advanced knowledge about technological tools, devices and tools used in histological studies | 4 |
| PLO07 | Bilgi - Kuramsal, Olgusal | Knows the importance of ethical rules and ethical principles. | 2 |
| PLO08 | Bilgi - Kuramsal, Olgusal | Has knowledge about planning of experimental studies and statistical methods. | |
| PLO09 | Bilgi - Kuramsal, Olgusal | Prepares and examines the tissues using electron microscopic, immunohistochemistry and special staining techniques. | |
| PLO10 | Bilgi - Kuramsal, Olgusal | Usestechnological tools, devices and equipment according to the level. | 3 |
| PLO11 | Bilgi - Kuramsal, Olgusal | Prepares experimental studies as a project. | 3 |
| PLO12 | Bilgi - Kuramsal, Olgusal | Presents the results of research in scientific meetings and publishes them in refereed journals. | |
| PLO13 | Bilgi - Kuramsal, Olgusal | Makes critical analysis, synthesis and evaluation related to the field. | 2 |
| PLO14 | Bilgi - Kuramsal, Olgusal | Carries out studies individually or as a team member and leads the necessary environments. | |
| PLO15 | Bilgi - Kuramsal, Olgusal | Bilimsel bir çalışmayı bağımsız olarak planlayıp, gerçekleştirerek istatistiksel analizlerini yapar. | |
| PLO16 | Beceriler - Bilişsel, Uygulamalı | Uses the principles of professional development and lifelong learning in their studies. | 3 |
| PLO17 | Beceriler - Bilişsel, Uygulamalı | Uses critical thinking, decision making and evidence-based medicine applications in their research. | |
| PLO18 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | By communicating effectively, discusses the issues related to the field and defends their views. | |
| PLO19 | Yetkinlikler - Öğrenme Yetkinliği | Works with relevant disciplines in the process of selecting, applying and interpreting appropriate methods for studies and establish functional communication. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Definition and terminology of cell culture | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 2 | Cell culture types; adherent and suspension cultures | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 3 | Cell culture types; adherent and suspension cultures 2 | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 4 | Secondary cultures and cell lines | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 5 | Mediums used in cell culture, sera, antibiotics | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 6 | The importance of sterilization in cell culture | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım, Gösteri |
| 7 | Contamination problems in cell culture laboratory | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım, Gösteri |
| 8 | Mid-Term Exam | Self-study, reading recommended references | Ölçme Yöntemleri: Yazılı Sınav |
| 9 | Freezing of cells in culture media | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 10 | The use of cell culture | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 11 | Immunohistochemical techniques in cell culture | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 12 | Cell viability tests | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım, Gösteri |
| 13 | seminar | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 14 | Microscope types used in cell culture | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 15 | Criteria for evaluation of cells produced in culture | Self-study, reading recommended references | Öğretim Yöntemleri: Anlatım |
| 16 | Term Exams | Self-study, reading recommended references | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | Ölçme Yöntemleri: Sözlü Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 3 | 42 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 3 | 42 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 7 | 7 |
| Final Exam | 1 | 18 | 18 |
| Total Workload (Hour) | 109 | ||
| Total Workload / 25 (h) | 4,36 | ||
| ECTS | 4 ECTS | ||