BB625 Ultra-Cold Conditions (Cryopreservation) Preservation Methods and Applications

6 ECTS - 2-2 Duration (T+A)- . Semester- 3 National Credit

Information

Code BB625
Name Ultra-Cold Conditions (Cryopreservation) Preservation Methods and Applications
Term 2024-2025 Academic Year
Semester . Semester
Duration (T+A) 2-2 (T-A) (17 Week)
ECTS 6 ECTS
National Credit 3 National Credit
Teaching Language Türkçe
Level Yüksek Lisans Dersi
Type Normal
Mode of study Yüz Yüze Öğretim
Catalog Information Coordinator Prof. Dr. YILDIZ KAÇAR


Course Goal / Objective

This course aims to teach the basic concepts and subjects of cryopreservation.

Course Content

The contents of this course include introduction to the historical development of cryopreservation techniques; slow cooling techniques; rapid cooling techniques; cold hardening, cryoprotectants (DMSO, ethylene glycol, sucrose, mannitol, sorbitol, etc.); vitrification technique and the preparation of vitrification solutions (PVS1, PVS2, PVS3, etc.); desiccation technique (silica gel and laminar flow cabinet); synthetic seed production (encapsulation); encapsulation dehydration; desiccation encapsulation; droplet method.

Course Precondition

no

Resources

Notes and presentations prepared within the scope of the course content.

Notes

Plant Cryopreservation Carla Benelli (Ed.)


Course Learning Outcomes

Order Course Learning Outcomes
LO01 Have knowledge about plant genetic resources and conservation.
LO02 Knows and applies different Croyopreservation methods.
LO03 Knows the issues of synthetic seeds and their use, comprehends the biotechnological importance.
LO04 Carrying out a study individually that requires professional knowledge in Cryopreservation
LO05 Entering database related to horticulture, utilizing those sources and self-updating.
LO06 Evaluating the knowledge and skills in horticulture by criticism and directing their own learning process
LO07 Presenting their studies, developments of their expertise topic and research results by orally, written and taking advantage of visual equipments
LO08 Obtaining data by scientific methods in order to solve a problem related to horticulture, establishing and interpreting the data by considering the scientific and ethical values


Relation with Program Learning Outcome

Order Type Program Learning Outcomes Level
PLO01 Bilgi - Kuramsal, Olgusal To be able to develop and deepen knowledge acquired in horticultural field based on undergraduate level competencies 1
PLO02 Bilgi - Kuramsal, Olgusal To be able to understand the interaction between horticultural field and related disciplines 2
PLO03 Bilgi - Kuramsal, Olgusal To be able to combine and interpret the theoretical and practical knowledge related to horticultural plants with the knowledge, data and findings obtained from different disciplines, to be able to create new knowledge and theories by synthesizing them by using the theoretical and practical knowledge related to horticultural plants in the field of expertise and supporting the current developments with quantitative and qualitative 2
PLO04 Bilgi - Kuramsal, Olgusal Solving problems by using research methods and solving cause and effect relationships 2
PLO05 Bilgi - Kuramsal, Olgusal To be able to conduct a study requiring expertise on horticultural plants independently 2
PLO06 Bilgi - Kuramsal, Olgusal To develop an analytical approach for solving unpredictable complex problems encountered in horticultural applications, to design the research process, to produce solutions by taking responsibility and to evaluate and defend the results obtained 1
PLO07 Bilgi - Kuramsal, Olgusal To lead the horticultural plants in an environment that requires solving problems
PLO08 Beceriler - Bilişsel, Uygulamalı Access to resources related to garden plants, benefiting from these resources and self-renewal 2
PLO09 Beceriler - Bilişsel, Uygulamalı To be able to evaluate the knowledge and skills acquired in the field of horticulture with a critical approach and to direct the learning process 2
PLO10 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği To be able to transfer his / her studies, developments in his / her field of expertise and research results using oral, written and visual tools 3
PLO11 Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği To be able to use advanced computer software and information and communication technologies as necessary in relation to horticultural plants
PLO12 Yetkinlikler - Öğrenme Yetkinliği To be able to collect data with scientific methods for solving problems related to horticultural plants, to be able to control and interpret the collected data by taking into consideration the social, scientific and ethical values.
PLO13 Yetkinlikler - Öğrenme Yetkinliği To be able to use assimilated information in the field of horticulture in interdisciplinary studies, explain it, transfer it to others, examine the results from a critical perspective


Week Plan

Week Topic Preparation Methods
1 Plant Genetic Resources and consevation, The historical development of cryopreservation techniques Reading the related lecture notes Öğretim Yöntemleri:
Anlatım, Tartışma, Gösteri
2 Slow cooling techniques Reading the related lecture notes Öğretim Yöntemleri:
Anlatım, Tartışma, Gösteri
3 Rapid cooling techniques Reading the related lecture notes Öğretim Yöntemleri:
Anlatım, Tartışma, Gösteri
4 Cold hardening Reading the related lecture notes Öğretim Yöntemleri:
Anlatım, Tartışma, Gösteri
5 Cryoprotectans Reading the related lecture notes Öğretim Yöntemleri:
Anlatım, Tartışma, Gösteri
6 Vitrification technique and preparation of solutions Reading the related lecture notes Öğretim Yöntemleri:
Anlatım, Tartışma, Gösteri
7 Desiccation technique Reading the related lecture notes Öğretim Yöntemleri:
Anlatım, Tartışma, Gösteri
8 Mid-Term Exam Reading the related lecture notes Ölçme Yöntemleri:
Yazılı Sınav
9 Production of synthetic seeds Reading the related lecture notes Öğretim Yöntemleri:
Anlatım, Tartışma, Gösteri
10 Encapsulation dehydration Reading the related lecture notes Öğretim Yöntemleri:
Anlatım, Tartışma, Gösteri
11 Encapsulation desiccation Reading the related lecture notes Öğretim Yöntemleri:
Anlatım, Tartışma, Gösteri
12 Encapsulation vitrification Reading the related lecture notes Öğretim Yöntemleri:
Anlatım, Tartışma, Gösteri
13 Cryopreservation of embriyogenic culture Reading the related lecture notes Öğretim Yöntemleri:
Anlatım, Tartışma, Gösteri
14 Cryopreservation of Bryophytes and fern Reading the related lecture notes Öğretim Yöntemleri:
Anlatım, Tartışma, Gösteri
15 Droplet method Reading the related lecture notes Öğretim Yöntemleri:
Anlatım, Tartışma, Gösteri
16 Term Exams Reading the related lecture notes Ölçme Yöntemleri:
Yazılı Sınav
17 Term Exams Reading the related lecture notes Ölçme Yöntemleri:
Yazılı Sınav


Student Workload - ECTS

Works Number Time (Hour) Workload (Hour)
Course Related Works
Class Time (Exam weeks are excluded) 14 4 56
Out of Class Study (Preliminary Work, Practice) 14 4 56
Assesment Related Works
Homeworks, Projects, Others 1 2 2
Mid-term Exams (Written, Oral, etc.) 1 12 12
Final Exam 1 28 28
Total Workload (Hour) 154
Total Workload / 25 (h) 6,16
ECTS 6 ECTS

Update Time: 06.05.2024 02:45