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Information
| Code | BFZ513 |
| Name | Flourescence imaging techniques in the cell |
| Term | 2024-2025 Academic Year |
| Semester | . Semester |
| Duration (T+A) | 3-2 (T-A) (17 Week) |
| ECTS | 5 ECTS |
| National Credit | 2 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Doç. Dr. FİGEN ÇİÇEK |
Course Goal / Objective
Learning the fluorescence imaging techniques in the cell and perform fluorescence imaging in the cell.
Course Content
Fluorescence and its physics principles. Chemical fluorescence probes and their cellular usages. Measurement of the basic ions with the fluorescent techniques in the cells Dynamic measurement of the cellular signalling pathways with fluorescent probes. Biological fluorescent probes and their cellular usages. Other techniques; FRET, Fluorescent life time microscopes and immunofluorescent techniques.
Course Precondition
The lesson has no prerequisites
Resources
Lecture notes (For Medical Faculty Students - İsmail Günay) Biophysics (Ferit Pehlivan) Biophysics (Gürbüz Çelebi) Internet Search
Notes
Internet Research
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Learns and applies the concept of fluorescence according to the basics of physics |
| LO02 | Learns various fluorescence imaging techniques. |
| LO03 | Learns to decide florophore, type of the florescent system, wavelength and filters according to the experimental system. |
| LO04 | Experimental data analysis and interpretations can be made |
| LO05 | Learns and applies intracellular ion imaging |
| LO06 | Measurement of dynamic ion concentration information in the intracellular region |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Learns basic information about the field | 2 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Sets up and works experimental setups in the laboratory | 2 |
| PLO03 | Bilgi - Kuramsal, Olgusal | Prepares a project proposal on its own | |
| PLO04 | Bilgi - Kuramsal, Olgusal | Plans an experimental study | 2 |
| PLO05 | Bilgi - Kuramsal, Olgusal | Reports the research result | 3 |
| PLO06 | Bilgi - Kuramsal, Olgusal | Interpret the research findings | 2 |
| PLO07 | Beceriler - Bilişsel, Uygulamalı | Has the analytical thinking mechanism related to the field, Reaches reliable information. Creates and writes a research project, works and researches based on ethical values. | |
| PLO08 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | She/he systematically transfers the theoretical knowledge she has researched to the audience. | |
| PLO09 | Yetkinlikler - Öğrenme Yetkinliği | Have a scientific thinking systematic in relation to their profession. | 3 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | The concept of fluorescence and its physics basis | Reads the related chapter from the textbook and lecture notes. | Öğretim Yöntemleri: Soru-Cevap |
| 2 | Biological fluorescence probes and their cellular usages | Reads the related chapter from the textbook and lecture notes. | Öğretim Yöntemleri: Gösteri |
| 3 | Chemical fluorescent dyes and their types and usages | Reads the related chapter from the textbook and lecture notes. | Öğretim Yöntemleri: Beyin Fırtınası |
| 4 | Fluorescent measurement systems and their working principles 1 Fluorimeters-1 | Reads the related chapter from the textbook and lecture notes. | Öğretim Yöntemleri: Tartışma, Gösteri |
| 5 | Fluorescent measurement systems and their working principles 2 Camera and confocal systems-2 | Reads the related chapter from the textbook and lecture notes. | Öğretim Yöntemleri: Gösterip Yaptırma |
| 6 | Ion imaging in the cell. | Reads the related chapter from the textbook and lecture notes. | Öğretim Yöntemleri: Tartışma |
| 7 | Calibration in the fluorescent measurements | Reads the related chapter from the textbook and lecture notes. | Öğretim Yöntemleri: Soru-Cevap, Tartışma |
| 8 | Mid-Term Exam | Mid-Term Exam | Ölçme Yöntemleri: Yazılı Sınav |
| 9 | Analysis study of experimental data | Reads the related chapter from the textbook and lecture notes | Öğretim Yöntemleri: Tartışma, Beyin Fırtınası |
| 10 | Measurement of dynamic ion concentrations in the celli | Reads the related chapter from the textbook and lecture notes | Öğretim Yöntemleri: Soru-Cevap |
| 11 | LASER as a light source | Reads the related chapter from the textbook and lecture notes | Öğretim Yöntemleri: Beyin Fırtınası, Bireysel Çalışma |
| 12 | Fluorescent meaurement of DNA and immunofluorescent techniques | Reads the related chapter from the textbook and lecture notes | Öğretim Yöntemleri: Benzetim, Problem Çözme |
| 13 | FRET and Fluoresence lifetime imaging microscopy | Reads the related chapter from the textbook and lecture notes | Öğretim Yöntemleri: Soru-Cevap, Tartışma |
| 14 | Flow cytometry | Reads the related chapter from the textbook and lecture notes | Öğretim Yöntemleri: Gösteri, Tartışma |
| 15 | A fluorescence system design project work | Reads the related chapter from the textbook and lecture notes | Öğretim Yöntemleri: Soru-Cevap, Alıştırma ve Uygulama |
| 16 | Term Exams-1 | To study exam | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams-2 | to study exam | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 3 | 42 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 4 | 56 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 5 | 5 |
| Final Exam | 1 | 15 | 15 |
| Total Workload (Hour) | 118 | ||
| Total Workload / 25 (h) | 4,72 | ||
| ECTS | 5 ECTS | ||