TÜRKÇE
ENGLISH
Information
| Code | BİO525 |
| Name | Cytogenetics (Z) |
| Term | 2024-2025 Academic Year |
| Semester | . Semester |
| Duration (T+A) | 2-3 (T-A) (17 Week) |
| ECTS | 5 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. AYFER PAZARBAŞI |
Course Goal / Objective
Demonstrating and presenting characteristics, mutations and cytogenetic analysis of chromosomes and pedigree analysis.
Course Content
Packaging of DNA and chromosome, characteristics, mutations and cytogenetic analysis of chromosomes, Pedigree analysis, Otosomal and sexual abnormalities, Genetic control of gender, Separation of chromosomes, Giant chromosomes, Barr body, Mendelian and non mendelian inheritance, Prenatal diagnosis and Genetic screening.
Course Precondition
To have taken the BIO-502 Basic Medical Genetics Course
Resources
Chromosome Biology Clinical Cytogenetics Medical Genetics
Notes
Basic principles in genetics
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | The student tells packaging of DNA and chromosome |
| LO02 | The student lists characteristics of chromosomes |
| LO03 | The student lists mutations of chromosomes |
| LO04 | The student distinguishes Cytogenetic analysis of chromosomes: lymphocyte culture and preparing metaphase plaque |
| LO05 | The student tells and applies karyotype analyses |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | To comprehend the structure and functions of cells and organelles which constitute the basic structure of life | |
| PLO02 | Bilgi - Kuramsal, Olgusal | To recognize the functions of tissue and organ systems | |
| PLO03 | Bilgi - Kuramsal, Olgusal | To examine the structure of genetic material | 4 |
| PLO04 | Bilgi - Kuramsal, Olgusal | To recognize genetic diseases and genes that cause these diseases | 2 |
| PLO05 | Bilgi - Kuramsal, Olgusal | To learn the structure of genes | 2 |
| PLO06 | Bilgi - Kuramsal, Olgusal | To define chromosome and gene mutations | 3 |
| PLO07 | Bilgi - Kuramsal, Olgusal | To classify heredity forms (autosomal dominant, autosomal recessive, gender-dependent dominant and recessive inheritance models) | 5 |
| PLO08 | Bilgi - Kuramsal, Olgusal | To use molecular and cytogenetic analysis | 4 |
| PLO09 | Bilgi - Kuramsal, Olgusal | To be able to explain the numerical and structural anomalies of autosomal and sex chromosomes | 4 |
| PLO10 | Bilgi - Kuramsal, Olgusal | To make DNA isolation from all kinds of tissues | 2 |
| PLO11 | Bilgi - Kuramsal, Olgusal | To prepare PCR | 2 |
| PLO12 | Bilgi - Kuramsal, Olgusal | To make and interpret agarose and polyacrylamide gel electrophoresis | 2 |
| PLO13 | Bilgi - Kuramsal, Olgusal | To measure the obtained DNA on the spectrophotometer | 2 |
| PLO14 | Bilgi - Kuramsal, Olgusal | To apply and interpret QF-PCR technique | 2 |
| PLO15 | Bilgi - Kuramsal, Olgusal | To make cell culture from blood, amnion, chorionic villus biopsy specimens and to obtain chromosome preparation | 4 |
| PLO16 | Bilgi - Kuramsal, Olgusal | To perform karyotype analysis of chromosome preparations and to recognize and interpret all human chromosomes | 4 |
| PLO17 | Beceriler - Bilişsel, Uygulamalı | To interpret a scientific article and to learn how to write a paper |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Packaging of DNA and chromosome | Literature review and reading | Öğretim Yöntemleri: Anlatım, Tartışma |
| 2 | Structure and properties of chromosomes | Scanning relevant literature and resources. | Öğretim Yöntemleri: Anlatım |
| 3 | Numerical irregularities of chromosomes | Reading related resources | Öğretim Yöntemleri: Alıştırma ve Uygulama, Anlatım, Tartışma |
| 4 | Structural irregularities of chromosomes | Reading relevant literature and resources | Öğretim Yöntemleri: Alıştırma ve Uygulama |
| 5 | Examining the methods of obtaining chromosomes | Reading relevant literature and resources | Öğretim Yöntemleri: Anlatım, Alıştırma ve Uygulama, Gösteri |
| 6 | Analyses of karyotype and pedigree | Literature review and reading | Öğretim Yöntemleri: Anlatım, Gösterip Yaptırma, Alıştırma ve Uygulama |
| 7 | autosomal chromosomal aberrations | Literature review and reading | Öğretim Yöntemleri: Anlatım, Tartışma |
| 8 | Mid-Term Exam | Öğretim Yöntemleri: Problem Çözme |
|
| 9 | Sex chromosome aberrations and genetic control of sex | Literature review and reading | Öğretim Yöntemleri: Anlatım, Tartışma |
| 10 | Chromosome separation mechanisms and genetics | Reading relevant literature and resources | Öğretim Yöntemleri: Anlatım, Alıştırma ve Uygulama |
| 11 | Methods of obtaining giant chromosome and Barr body | Reading relevant literature and resources | Öğretim Yöntemleri: Anlatım, Alıştırma ve Uygulama |
| 12 | Mendelian Inheritance model and its applications | Reading relevant literature and resources | Öğretim Yöntemleri: Anlatım, Tartışma |
| 13 | Non mendelian inheritance model | Reading relevant literature and resources | Öğretim Yöntemleri: Anlatım, Alıştırma ve Uygulama |
| 14 | Prenatal Diagnosis Methods and applications | Reading relevant literature and resources | Öğretim Yöntemleri: Tartışma, Alıştırma ve Uygulama |
| 15 | Applications of Genetic Screening methods | Literature review and reading | Öğretim Yöntemleri: Anlatım, Alıştırma ve Uygulama, Deney / Laboratuvar |
| 16 | Term Exams | Preparing multiple choice and classical tests | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Evaluation of the exam | Yöntem Seçilmemiş |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 3 | 42 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 5 | 70 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 1 | 8 | 8 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 5 | 5 |
| Final Exam | 1 | 3 | 3 |
| Total Workload (Hour) | 128 | ||
| Total Workload / 25 (h) | 5,12 | ||
| ECTS | 5 ECTS | ||