TÜRKÇE
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Information
| Unit | INSTITUTE OF MEDICAL SCIENCES |
| MEDICAL BIOCHEMISTRY (MEDICINE) (MASTER) (WITH THESIS) | |
| Code | BİK551 |
| Name | METHODS OF ISOLATION AND STORAGE OF NUCLEİC ACİDS |
| Term | 2025-2026 Academic Year |
| Term | Fall and Spring |
| Duration (T+A) | 3-2 (T-A) (17 Week) |
| ECTS | 7 ECTS |
| National Credit | 4 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | |
| Course Instructor |
The current term course schedule has not been prepared yet.
|
Course Goal / Objective
The aim of this course is to provide scientific and technical knowledge on the principles of DNA and RNA isolation, commonly used methods, quality control processes, and safe long-term storage of isolated products.
Course Content
The course covers the biochemical characteristics of nucleic acids, cell-type specific isolation techniques, manual and kit-based methods, sources of contamination, quality control techniques, and short- and long-term storage conditions of the isolated products.
Course Precondition
pass the exam
Resources
Sambrook & Russell – Molecular Cloning: A Laboratory Manual
Notes
Ausubel et al. – Current Protocols in Molecular Biology
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Identifies structural features of DNA and RNA |
| LO02 | Explains nucleic acid isolation from various samples |
| LO03 | Compares kit-based and manual isolation methods |
| LO04 | Interprets quality control (QC) measurements |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Yetkinlikler - Alana Özgü Yetkinlik | Explain the structure and function of the basic tissues of the body. | 1 |
| PLO02 | Belirsiz | Explain the structure and functions of the cell, their physiological mechanisms and bioelectrical events. | 1 |
| PLO03 | Belirsiz | Explain the structure and functions of biochemical molecules involved in metabolism. | 1 |
| PLO04 | Belirsiz | Know the structure and function of genetic material (DNA and RNA), gene expression, genome structure, nucleic acid metabolism and the use of DNA in medicine, the basic principles of heredity. |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Course Introduction and Basic Concepts | Structural Features of DNA and RNA | Öğretim Yöntemleri: Anlatım |
| 2 | Nucleic Acid Functions in Cells | Sample Types for Isolation | Öğretim Yöntemleri: Anlatım |
| 3 | Principles of Cell and Tissue Lysis | Manual Isolation Techniques | Öğretim Yöntemleri: Anlatım |
| 4 | Organic Extraction (Phenol/Chloroform) | Inorganic Methods and Salt Precipitation | Öğretim Yöntemleri: Anlatım |
| 5 | Silica Membrane Technology | Magnetic Bead-Based Methods | Öğretim Yöntemleri: Anlatım |
| 6 | Automated Extraction Systems | RNA-Specific Methods | Öğretim Yöntemleri: Anlatım |
| 7 | DNase and RNase Control | Post-Isolation Cleanup Steps | Öğretim Yöntemleri: Anlatım |
| 8 | Mid-Term Exam | Ölçme Yöntemleri: Sözlü Sınav |
|
| 9 | A260/A280 and A260/A230 Absorbance Ratios | Quality Evaluation by Agarose Gel Electrophoresis | Öğretim Yöntemleri: Anlatım |
| 10 | Bioanalyzer and TapeStation Systems | Functional QC via PCR and qPCR | Öğretim Yöntemleri: Anlatım |
| 11 | DNA/RNA Stability: Contributing Factors | Short-Term Storage Conditions | Öğretim Yöntemleri: Anlatım |
| 12 | Long-Term Storage Conditions | Lyophilization of Isolated Products | Öğretim Yöntemleri: Anlatım |
| 13 | Freezer and Liquid Nitrogen Storage Systems | Freeze-Thaw Cycle Risks | Öğretim Yöntemleri: Anlatım |
| 14 | Laboratory Contamination and Prevention | Preparing Isolation Checklists | Öğretim Yöntemleri: Anlatım |
| 15 | Clinical vs. Research-Based Isolation | Comparative Review of Commercial Kits | Öğretim Yöntemleri: Anlatım |
| 16 | Term Exams | Ölçme Yöntemleri: Yazılı Sınav |
|
| 17 | Term Exams | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 3 | 42 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 7 | 98 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 15 | 15 |
| Final Exam | 1 | 30 | 30 |
| Total Workload (Hour) | 185 | ||
| Total Workload / 25 (h) | 7,40 | ||
| ECTS | 7 ECTS | ||