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Information
| Unit | INSTITUTE OF MEDICAL SCIENCES |
| BIOPHYSICS (MEDICINE) (MASTER) (WITH THESIS) | |
| Code | BFZ514 |
| Name | Biophysical Approach-Based Molecular Analysis |
| Term | 2026-2027 Academic Year |
| Term | Fall and Spring |
| Duration (T+A) | 2-2 (T-A) (17 Week) |
| ECTS | 5 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Belirsiz |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Doç. Dr. ÇAĞIL COŞKUN |
| Course Instructor |
The current term course schedule has not been prepared yet.
|
Course Goal / Objective
To provide fundamental knowledge for applying molecular techniques and interpreting their results by understanding their biophysical principles.
Course Content
Introduction to molecular biophysics: experimental approach, Physical properties of biological macromolecules, Cell lysis and the biophysics of membranes, DNA isolation and its biophysical principles: electrostatics and solubility, RNA isolation and its biophysical principles, Nucleic acid quantification: optical principles, Biophysics of PCR 1–2, Electrophoresis: electric field–mobility relationship, Biophysics of qPCR 1–2, Protein isolation and SDS-PAGE: denaturation and molecular separation, Biophysical principles of Western blotting, Experimental errors and biological variations.
Course Precondition
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Resources
Biophysics and Molecular Biology Tools and Techniques Pranav Kumar
Notes
Biophysics and Molecular Biology Tools and Techniques Pranav Kumar
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Acquires comprehensive knowledge of molecular biophysics |
| LO02 | Acquires knowledge of the physical foundations of biological macromolecules |
| LO03 | Acquires comprehensive knowledge of the principles governing DNA, RNA, and protein isolation and their biophysical basis |
| LO04 | Acquires comprehensive knowledge of key molecular techniques (PCR, qPCR, Western blot) and their biophysical basis |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Learns basic information about the field | 5 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Sets up and works experimental setups in the laboratory | 5 |
| PLO03 | Bilgi - Kuramsal, Olgusal | Prepares a project proposal on its own | |
| PLO04 | Bilgi - Kuramsal, Olgusal | Plans an experimental study | 5 |
| PLO05 | Bilgi - Kuramsal, Olgusal | Reports the research result | |
| PLO06 | Bilgi - Kuramsal, Olgusal | Interpret the research findings | 5 |
| PLO07 | Beceriler - Bilişsel, Uygulamalı | Has the analytical thinking mechanism related to the field, Reaches reliable information. Creates and writes a research project, works and researches based on ethical values. | 4 |
| PLO08 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | She/he systematically transfers the theoretical knowledge she has researched to the audience. | |
| PLO09 | Yetkinlikler - Öğrenme Yetkinliği | Have a scientific thinking systematic in relation to their profession. | 4 |
| PLO10 | Bilgi - Kuramsal, Olgusal | Kendi başına bir proje önerisi hazırlayabilmek | 4 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Introduction to Molecular Biophysics: An Experimental Approach | Reading of the relevant section from the sources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 2 | Physical Properties of Biological Macromolecules | Reading of the relevant section from the sources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 3 | Biophysical Aspects of Cell Lysis and Membranes | Reading of the relevant section from the sources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 4 | DNA Isolation and Its Biophysical Principles: Electrostatics and Solubility | Reading of the relevant section from the sources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 5 | Biophysical Principles of RNA Isolation | Reading of the relevant section from the sources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 6 | Quantification of Nucleic Acids: Optical Principles | Reading of the relevant section from the sources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 7 | Biophysics of PCR -1 | Reading of the relevant section from the sources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 8 | Mid-Term Exam | Ölçme Yöntemleri: Yazılı Sınav |
|
| 9 | Biophysics of PCR -2 | Reading of the relevant section from the sources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 10 | Electrophoresis: Electric Field–Mobility Relationship | Reading of the relevant section from the sources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 11 | Biophysics of qPCR -1 | Reading of the relevant section from the sources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 12 | Biophysics of qPCR -2 | Reading of the relevant section from the sources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 13 | Protein Isolation and SDS-PAGE: Denaturation and Molecular Separation | Reading of the relevant section from the sources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 14 | Biophysical Principles of Western Blotting | Reading of the relevant section from the sources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 15 | Experimental Errors and Biological Variability | Reading of the relevant section from the sources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 16 | Term Exams | Ölçme Yöntemleri: Yazılı Sınav |
|
| 17 | Term Exams | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 4 | 56 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 3 | 42 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 5 | 5 |
| Final Exam | 1 | 15 | 15 |
| Total Workload (Hour) | 118 | ||
| Total Workload / 25 (h) | 4,72 | ||
| ECTS | 5 ECTS | ||