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Information
| Unit | ABDİ SÜTCÜ HEALTH SERVICES VOCATIONAL SCHOOL |
| Code | SHS181 |
| Name | Molecular Analyses in Diagnosis |
| Term | 2026-2027 Academic Year |
| Semester | 1. Semester |
| Duration (T+A) | 2-0 (T-A) (17 Week) |
| ECTS | 3 ECTS |
| National Credit | 2 National Credit |
| Teaching Language | Türkçe |
| Level | Belirsiz |
| Type | Normal |
| Label | E Elective |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Doç. Dr. SEDEFGÜL YÜZBAŞIOĞLU ARIYÜREK |
| Course Instructor |
The current term course schedule has not been prepared yet.
|
Course Goal / Objective
The aim of this course is for students to gain fundamental knowledge about molecular analysis techniques used for diagnostic purposes in medical laboratories, as well as pre-analytical processes and techniques.
Course Content
This course covers the isolation and analysis of molecular analysis materials, PCR technology, the development and application areas of molecular analyses, traditional molecular analysis techniques, and the contributions of technological developments to molecular analysis techniques.
Course Precondition
None
Resources
Tietz Textbook of Clinical Chemistry and Molecular Diagnostics, Carl A. Burtis,Edward R. Ashwood, David E. Bruns, 2012
Notes
Khan Academy videos, Open digital resources
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | It describes the structure and properties of DNA. |
| LO02 | Explains DNA isolation. |
| LO03 | Describes the structure and properties of mtDNA. |
| LO04 | Explains polymerase chain reaction (PCR). |
| LO05 | Explains PCR-based molecular analysis methods. |
| LO06 | Explains blotting techniques. |
| LO07 | It explains array technologies. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Explains the basis of human normal structure and function | |
| PLO02 | Bilgi - Kuramsal, Olgusal | Defines disinfection, sterilization and hygiene. | |
| PLO03 | Bilgi - Kuramsal, Olgusal | Recognizes laboratory tools- materials, and measurement methods. | |
| PLO04 | Bilgi - Kuramsal, Olgusal | Explains the procedures to be performed in these phases using detailed information on the preanalytical, analytical and postanalytical phases of measurements. | 2 |
| PLO05 | Beceriler - Bilişsel, Uygulamalı | Receives and accepts biological materials taken from the patient, prepares them for analysis and ensures that they are preserved under appropriate conditions if necessary. | 2 |
| PLO06 | Beceriler - Bilişsel, Uygulamalı | Performs internal and external quality control studies of the analyzes, analyzes patient samples after applying daily control procedures, checks the results and reports to the laboratory specialist. | |
| PLO07 | Beceriler - Bilişsel, Uygulamalı | Performs the calibration and maintenance of laboratory equipments, have them to be calibrated and maintained and monitored by authorized persons as needed, so that they work most efficiently | 2 |
| PLO08 | Beceriler - Bilişsel, Uygulamalı | Determines inaduquaciesis in the laboratory | |
| PLO09 | Bilgi - Kuramsal, Olgusal | It provides coordination by reporting critical values to the clinician in the most appropriate and rapid way. | |
| PLO10 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | After graduation, he/she develops himself/herself and his/her career by following technological developments and critically evaluating the information he/she has learned with his/her own knowledge. | |
| PLO11 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Having professional and ethical responsibility, they communicate well with colleagues, patients and physicians. | |
| PLO12 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Uses at least one foreign language to follow developments in his field and to communicate with colleagues adequately. | |
| PLO13 | Yetkinlikler - Öğrenme Yetkinliği | Takes measures regarding health, biosafety and occupational safety in the work environment. | 2 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Molecular analysis materials | Reading from resources | Öğretim Yöntemleri: Anlatım, Gösteri |
| 2 | DNA isolation and quantification | Reading from sources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Gösteri |
| 3 | PCR technology | Reading from sources | Öğretim Yöntemleri: Anlatım, Tartışma, Beyin Fırtınası |
| 4 | What is a primer | Reading from sources | Öğretim Yöntemleri: Anlatım, Tartışma, Gösteri |
| 5 | PCR-based molecular analysis techniques | Reading from sources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Alıştırma ve Uygulama, Gösteri |
| 6 | Traditional molecular analysis techniques | Reading from sources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Gösteri, Örnek Olay |
| 7 | Mutation analyses | Reading from sources | Öğretim Yöntemleri: Anlatım, Tartışma, Gösteri, Örnek Olay |
| 8 | Mid-Term Exam | Preparing for the exam from resources | Ölçme Yöntemleri: Yazılı Sınav |
| 9 | Validation of analyses | Reading from resources | Öğretim Yöntemleri: Anlatım, Tartışma, Gösteri, Örnek Olay |
| 10 | Interpretation of mutation analyses | Reading from resources | Öğretim Yöntemleri: Anlatım, Tartışma, Örnek Olay |
| 11 | Blotting techniques | Reading from resources | Öğretim Yöntemleri: Anlatım, Gösteri |
| 12 | Southern/Northern/Western blotting analyses | Reading from resources | Öğretim Yöntemleri: Anlatım, Tartışma, Gösteri |
| 13 | Array technologies | Reading from resources | Öğretim Yöntemleri: Anlatım, Tartışma, Gösteri, Beyin Fırtınası |
| 14 | Use of microarrays in medicine | Reading from resources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Gösteri, Beyin Fırtınası |
| 15 | Probe design | Reading from sources | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Bireysel Çalışma |
| 16 | Term Exams | Preparing for exams from sources | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | Preparing for exams from sources | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 2 | 28 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 2 | 28 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 5 | 5 |
| Final Exam | 1 | 10 | 10 |
| Total Workload (Hour) | 71 | ||
| Total Workload / 25 (h) | 2,84 | ||
| ECTS | 3 ECTS | ||