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Information
| Unit | FACULTY OF PHARMACY |
| PHARMACY PR. | |
| Code | ECS313 |
| Name | Chromatographic Methods |
| Term | 2026-2027 Academic Year |
| Semester | 5. Semester |
| Duration (T+A) | 2-0 (T-A) (17 Week) |
| ECTS | 2 ECTS |
| National Credit | 2 National Credit |
| Teaching Language | Türkçe |
| Level | Belirsiz |
| Type | Normal |
| Label | E Elective |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Dr. Öğr. Üyesi SAMET POYRAZ |
| Course Instructor |
The current term course schedule has not been prepared yet.
|
Course Goal / Objective
The aim of this course is to provide students with the theoretical foundations, working principles, and pharmaceutical applications of chromatographic separation techniques. Within this scope, students are expected to compare different chromatographic methods, select appropriate techniques for specific analyses, and interpret chromatographic data. Additionally, the course aims to introduce the fundamental concepts and applications of commonly used techniques such as gas chromatography (GC), high-performance liquid chromatography (HPLC), and advanced chromatographic methods.
Course Content
Basic principles of chromatography, separation mechanisms, and chromatographic parameters; classification of chromatographic methods; column and planar chromatography techniques; gas chromatography (GC) and its applications; high-performance liquid chromatography (HPLC) and its working principles; detector systems; advanced chromatographic techniques (HILIC, ion chromatography, size exclusion chromatography, supercritical fluid chromatography); applications of chromatographic methods in pharmaceutical analysis and interpretation of chromatographic data.
Course Precondition
There are no prerequisites for this course. However, basic knowledge of analytical chemistry and general chemistry is recommended.
Resources
Course notes consist of lecture slides prepared by the instructor, in-class explanations, and compiled materials from selected scientific sources. Gündüz, T. Instrumental Analysis, Gazi Publishing Skoog, D. A., Holler, F. J., Crouch, S. R. Principles of Analytical Chemistry Skoog, D. A., Holler, F. J., Nieman, T. A. Principles of Instrumental Analysis Poole, C. F. Liquid Chromatography: Fundamentals and Instrumentation, Elsevier
Notes
Recent scientific articles on chromatographic applications Review papers in pharmaceutical analysis International databases (ScienceDirect, PubMed, etc.)
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Explain the fundamental principles and separation mechanisms of chromatography. |
| LO02 | Classify chromatographic techniques (column, TLC, GC, HPLC, etc.). |
| LO03 | Describe the components and working principles of gas chromatography (GC). |
| LO04 | Explain the system and separation mechanisms of high-performance liquid chromatography (HPLC). |
| LO05 | Interpret chromatographic parameters such as retention time, resolution, selectivity, and theoretical plates. |
| LO06 | Compare the advantages and limitations of different chromatographic techniques. |
| LO07 | Select an appropriate chromatographic method for a given analytical purpose. |
| LO08 | Identify chromatographic detector systems (UV, PDA, MS) and explain their applications. |
| LO09 | Describe the basic principles of advanced chromatographic techniques (HILIC, ion chromatography, SEC, SFC). |
| LO10 | Evaluate and interpret chromatographic data (chromatograms). |
| LO11 | Explain the applications of chromatographic techniques in pharmaceutical analysis. |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | Have sufficient knowledge in pharmaceutical field and develop the knowledge. | 4 |
| PLO02 | Bilgi - Kuramsal, Olgusal | Comprehends professional knowledge with ethical responsibility through current laws and legislation. | |
| PLO03 | Beceriler - Bilişsel, Uygulamalı | Provides consultancy service to patients, community and health care personnel and evaluates advanced knowledge and skills with a critical approach. | 2 |
| PLO04 | Beceriler - Bilişsel, Uygulamalı | Possessing the knowledge of historical and current developments in the field of Pharmacy, monitoring, evaluation and application of scientific and evidence-based data, and adopting lifelong learning. | 4 |
| PLO05 | Beceriler - Bilişsel, Uygulamalı | Have the knowledge and skills to help provide patient-oriented pharmacy applications and individualized pharmaceutical care by learning the basics of Clinical Pharmacy, | |
| PLO06 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | Takes a role in the evaluation and analysis of the events and cases related to the development of pharmacy profession and education. | 4 |
| PLO07 | Yetkinlikler - Öğrenme Yetkinliği | Integrates and develops professional knowledge via different disciplines and uses the acquired knowledge to improve the health level and quality of life of the community. | 3 |
| PLO08 | Yetkinlikler - Öğrenme Yetkinliği | Use effectively the concepts and applications of basic sciences and basic medical sciences in the field of medicine and pharmacy and update the information. | 5 |
| PLO09 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Communicates effectively in writing and speaking. | 2 |
| PLO10 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Practices the profession in accordance with Atatürk's principles and reforms, equipped with universal knowledge, uses Turkish correctly and knows at least one foreign language. | |
| PLO11 | Yetkinlikler - İletişim ve Sosyal Yetkinlik | Be an example to society withexternal appearance, demeanor, attitude and behavior for the other professionals and society and with professional identity | |
| PLO12 | Yetkinlikler - Alana Özgü Yetkinlik | Have knowledge about health policies and health economics, pharmacy management and define the problems in pharmaceutical field. | |
| PLO13 | Yetkinlikler - Alana Özgü Yetkinlik | Interprets information about current health and treatment problems and attends meeting on related matters . Plays an active role in community health. | 2 |
| PLO14 | Yetkinlikler - Alana Özgü Yetkinlik | Acquires basic information about diseases, makes decisions about the drugs used in treatment in accordance with rational drug use. | 2 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | Chromatography theory and basic concepts | Brief reading on fundamental principles of chromatography | Öğretim Yöntemleri: Anlatım |
| 2 | Chromatographic methods and their classification | General research on types of chromatography | Öğretim Yöntemleri: Anlatım |
| 3 | Column chromatography | Investigation of stationary phases and elution techniques | Öğretim Yöntemleri: Anlatım |
| 4 | Thin layer chromatography (TLC) | Reading on Rf values and TLC applications | Öğretim Yöntemleri: Anlatım |
| 5 | Gas chromatography (GC) | Review of GC instrumentation components | Öğretim Yöntemleri: Anlatım |
| 6 | Applications of gas chromatography (GC) | Examples of volatile compound analysis | Öğretim Yöntemleri: Anlatım |
| 7 | High-performance liquid chromatography (HPLC) | Review of HPLC system components | Öğretim Yöntemleri: Anlatım |
| 8 | Mid-Term Exam | Ölçme Yöntemleri: Yazılı Sınav |
|
| 9 | Applications of HPLC | Reading on HPLC applications in pharmaceutical analysis | Öğretim Yöntemleri: Anlatım |
| 10 | Supercritical fluid chromatography (SFC) | Review of supercritical fluid concept | Öğretim Yöntemleri: Anlatım |
| 11 | Ion chromatography | Reading on ion exchange mechanisms | Öğretim Yöntemleri: Anlatım |
| 12 | Size exclusion chromatography (SEC) | Principles of size-based separation | Öğretim Yöntemleri: Anlatım |
| 13 | HILIC and advanced techniques | Reading on analysis of polar compounds | Öğretim Yöntemleri: Anlatım |
| 14 | Current applications in chromatography | Research on LC-MS applications | Öğretim Yöntemleri: Anlatım |
| 15 | General evaluation | Review of previous topics | Öğretim Yöntemleri: Anlatım |
| 16 | Term Exams | Ölçme Yöntemleri: Yazılı Sınav |
|
| 17 | Term Exams | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 2 | 28 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 1 | 14 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 4 | 4 |
| Final Exam | 1 | 4 | 4 |
| Total Workload (Hour) | 50 | ||
| Total Workload / 25 (h) | 2,00 | ||
| ECTS | 2 ECTS | ||