TÜRKÇE
ENGLISH
Information
| Code | BB584 |
| Name | Cross breeding in Citrus |
| Term | 2024-2025 Academic Year |
| Semester | . Semester |
| Duration (T+A) | 3-0 (T-A) (17 Week) |
| ECTS | 6 ECTS |
| National Credit | 3 National Credit |
| Teaching Language | Türkçe |
| Level | Yüksek Lisans Dersi |
| Type | Normal |
| Mode of study | Yüz Yüze Öğretim |
| Catalog Information Coordinator | Prof. Dr. TURGUT YEŞİLOĞLU |
Course Goal / Objective
The aims this course are to teach classical and somatic hybridization techniques in citrus; citrus flower biology, breeding strategy and aspects of hybridization breeding; important factors affecting the success of breeding; structure of the cell wall; protoplast isolation techniques (mechanical and chemical); explant resources, washing solutions used in enzymatic isolation; enzymes and their mixtures, washing and purifying the protoplast; determining the size and efficiency of protoplast; protoplast fusion methods (chemical, mechanical and electro-fusion); protoplast fusion and culturing the fused cells.
Course Content
The main objectives of this course are classical and somatic hybridization techniques in citrus; citrus flower biology, breeding strategy and aspects of hybridization breeding; important factors affecting the success of breeding; possible usage of somatic hybridization in regards to scion and rootstock breeding, hybridization breeding process, solutions for the problems in hybridization breeding.
Course Precondition
None
Resources
Lecture papers
Notes
Slide, Current papers
Course Learning Outcomes
| Order | Course Learning Outcomes |
|---|---|
| LO01 | Knows classical and somatic hybridization in citrus scion and rootstock breeding |
| LO02 | Knows enzymatic and mechanical protoplast isolation methods and protoplast fusion techniques |
| LO03 | Gains theorical and practical knowledge on related research techniques |
| LO04 | Gains skills for applying research results and theorical knowledge to practice |
Relation with Program Learning Outcome
| Order | Type | Program Learning Outcomes | Level |
|---|---|---|---|
| PLO01 | Bilgi - Kuramsal, Olgusal | To be able to develop and deepen knowledge acquired in horticultural field based on undergraduate level competencies | 3 |
| PLO02 | Bilgi - Kuramsal, Olgusal | To be able to understand the interaction between horticultural field and related disciplines | |
| PLO03 | Bilgi - Kuramsal, Olgusal | To be able to combine and interpret the theoretical and practical knowledge related to horticultural plants with the knowledge, data and findings obtained from different disciplines, to be able to create new knowledge and theories by synthesizing them by using the theoretical and practical knowledge related to horticultural plants in the field of expertise and supporting the current developments with quantitative and qualitative | |
| PLO04 | Bilgi - Kuramsal, Olgusal | Solving problems by using research methods and solving cause and effect relationships | 2 |
| PLO05 | Bilgi - Kuramsal, Olgusal | To be able to conduct a study requiring expertise on horticultural plants independently | 2 |
| PLO06 | Bilgi - Kuramsal, Olgusal | To develop an analytical approach for solving unpredictable complex problems encountered in horticultural applications, to design the research process, to produce solutions by taking responsibility and to evaluate and defend the results obtained | 3 |
| PLO07 | Bilgi - Kuramsal, Olgusal | To lead the horticultural plants in an environment that requires solving problems | 3 |
| PLO08 | Beceriler - Bilişsel, Uygulamalı | Access to resources related to garden plants, benefiting from these resources and self-renewal | 2 |
| PLO09 | Beceriler - Bilişsel, Uygulamalı | To be able to evaluate the knowledge and skills acquired in the field of horticulture with a critical approach and to direct the learning process | 3 |
| PLO10 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | To be able to transfer his / her studies, developments in his / her field of expertise and research results using oral, written and visual tools | 2 |
| PLO11 | Yetkinlikler - Bağımsız Çalışabilme ve Sorumluluk Alabilme Yetkinliği | To be able to use advanced computer software and information and communication technologies as necessary in relation to horticultural plants | |
| PLO12 | Yetkinlikler - Öğrenme Yetkinliği | To be able to collect data with scientific methods for solving problems related to horticultural plants, to be able to control and interpret the collected data by taking into consideration the social, scientific and ethical values. | |
| PLO13 | Yetkinlikler - Öğrenme Yetkinliği | To be able to use assimilated information in the field of horticulture in interdisciplinary studies, explain it, transfer it to others, examine the results from a critical perspective | 3 |
Week Plan
| Week | Topic | Preparation | Methods |
|---|---|---|---|
| 1 | General information about classical and somatic hybridization techniques in citrus | Reading related chapters of recommended resources. | Öğretim Yöntemleri: Anlatım, Tartışma |
| 2 | Flower structure and reproductive biology in citrus | Reading related chapters of recommended resources. | Öğretim Yöntemleri: Anlatım, Soru-Cevap, Tartışma |
| 3 | Breeding strategy and the importance of hybridization breeding in citrus | Reading related chapters of recommended resources. | Öğretim Yöntemleri: Soru-Cevap, Tartışma |
| 4 | Scion breeding by classical hybridization in citrus | Reading related chapters of recommended resources. | Öğretim Yöntemleri: Anlatım, Tartışma |
| 5 | The aspects and important points in orange and mandarin variety development | Reading related chapters of recommended resources. | Öğretim Yöntemleri: Anlatım, Tartışma |
| 6 | The aspects and important points in lemon and grapefruit variety development | Reading related chapters of recommended resources. | Öğretim Yöntemleri: Soru-Cevap, Tartışma, Anlatım |
| 7 | Rootstock breeding by classical hybridization in citrus | Reading related chapters of recommended resources. | Öğretim Yöntemleri: Anlatım |
| 8 | Mid-Term Exam | Reading related chapters of recommended resources. | Ölçme Yöntemleri: Ödev |
| 9 | Protoplast isolation, aims and scopes of application | Reading related chapters of recommended resources. | Öğretim Yöntemleri: Anlatım, Tartışma, Soru-Cevap |
| 10 | Mechanic and enzymatic methods in protoplast isolation | Reading related chapters of recommended resources. | Öğretim Yöntemleri: Anlatım, Tartışma, Soru-Cevap |
| 11 | Explant resources, growing conditions of donor plant, washing and enzym solutions in protoplast isolation | Reading related chapters of recommended resources. | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 12 | Washing, purifying and measuring the size and efficiency of protoplasts. | Reading related chapters of recommended resources. | Öğretim Yöntemleri: Anlatım, Tartışma, Soru-Cevap |
| 13 | Protoplast fusion methods (chemical, mechanic and electrofusion), protoplast fusion and conditions for culturing the fused cells | Reading related chapters of recommended resources. | Öğretim Yöntemleri: Anlatım |
| 14 | Possible usages of classical and somatic hybridization methods in scion breeding | Reading related chapters of recommended resources. | Öğretim Yöntemleri: Anlatım, Soru-Cevap |
| 15 | Possible usages of classical and somatic hybridization methods in rootstock breeding | Reading related chapters of recommended resources. | Öğretim Yöntemleri: Anlatım, Tartışma, Soru-Cevap |
| 16 | Term Exams | Reading related chapters of recommended resources. | Ölçme Yöntemleri: Yazılı Sınav |
| 17 | Term Exams | Reading related chapters of recommended resources. | Ölçme Yöntemleri: Yazılı Sınav |
Student Workload - ECTS
| Works | Number | Time (Hour) | Workload (Hour) |
|---|---|---|---|
| Course Related Works | |||
| Class Time (Exam weeks are excluded) | 14 | 3 | 42 |
| Out of Class Study (Preliminary Work, Practice) | 14 | 5 | 70 |
| Assesment Related Works | |||
| Homeworks, Projects, Others | 0 | 0 | 0 |
| Mid-term Exams (Written, Oral, etc.) | 1 | 15 | 15 |
| Final Exam | 1 | 30 | 30 |
| Total Workload (Hour) | 157 | ||
| Total Workload / 25 (h) | 6,28 | ||
| ECTS | 6 ECTS | ||